Antibodies against trimeric S glycoprotein protect hamsters against SARS-CoV challenge despite their capacity to mediate FcgammaRII-dependent entry into B cells in vitro.
Identifieur interne : 003771 ( Main/Exploration ); précédent : 003770; suivant : 003772Antibodies against trimeric S glycoprotein protect hamsters against SARS-CoV challenge despite their capacity to mediate FcgammaRII-dependent entry into B cells in vitro.
Auteurs : Yiu Wing Kam [République populaire de Chine] ; François Kien ; Anjeanette Roberts ; Yan Chung Cheung ; Elaine W. Lamirande ; Leatrice Vogel ; Shui Ling Chu ; Jane Tse ; Jeannette Guarner ; Sherif R. Zaki ; Kanta Subbarao ; Malik Peiris ; Béatrice Nal ; Ralf AltmeyerSource :
- Vaccine [ 0264-410X ] ; 2007.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (immunologie), Cricetinae, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (immunologie), Immunité muqueuse, Immunoglobuline A (métabolisme), Immunoglobuline G (métabolisme), Lymphocytes B (métabolisme), Protéines de l'enveloppe virale (immunologie), Relation dose-effet des médicaments, Récepteurs du fragment Fc des IgG (métabolisme), Souris, Syndrome respiratoire aigu sévère (), Virus du SRAS (immunologie).
- MESH :
- immunologie : Anticorps antiviraux, Glycoprotéines membranaires, Protéines de l'enveloppe virale, Virus du SRAS.
- métabolisme : Immunoglobuline A, Immunoglobuline G, Lymphocytes B, Récepteurs du fragment Fc des IgG.
- Animaux, Cricetinae, Glycoprotéine de spicule des coronavirus, Immunité muqueuse, Relation dose-effet des médicaments, Souris, Syndrome respiratoire aigu sévère.
English descriptors
- KwdEn :
- Animals, Antibodies, Viral (immunology), B-Lymphocytes (metabolism), Cricetinae, Dose-Response Relationship, Drug, Immunity, Mucosal, Immunoglobulin A (metabolism), Immunoglobulin G (metabolism), Membrane Glycoproteins (immunology), Mice, Receptors, IgG (metabolism), SARS Virus (immunology), Severe Acute Respiratory Syndrome (prevention & control), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (immunology).
- MESH :
- chemical , immunology : Antibodies, Viral, Membrane Glycoproteins, Viral Envelope Proteins.
- immunology : SARS Virus.
- metabolism : B-Lymphocytes, Immunoglobulin A, Immunoglobulin G, Receptors, IgG.
- prevention & control : Severe Acute Respiratory Syndrome.
- Animals, Cricetinae, Dose-Response Relationship, Drug, Immunity, Mucosal, Mice, Spike Glycoprotein, Coronavirus.
Abstract
Vaccine-induced antibodies can prevent or, in the case of feline infectious peritonitis virus, aggravate infections by coronaviruses. We investigated whether a recombinant native full-length S-protein trimer (triSpike) of severe acute respiratory syndrome coronavirus (SARS-CoV) was able to elicit a neutralizing and protective immune response in animals and analyzed the capacity of anti-S antibodies to mediate antibody-dependent enhancement (ADE) of virus entry in vitro and enhancement of replication in vivo. SARS-CoV-specific serum and mucosal immunoglobulins were readily detected in immunized animals. Serum IgG blocked binding of the S-protein to the ACE2 receptor and neutralized SARS-CoV infection in vitro. Entry into human B cell lines occurred in a FcgammaRII-dependent and ACE2-independent fashion indicating that ADE of virus entry is a novel cell entry mechanism of SARS-CoV. Vaccinated animals showed no signs of enhanced lung pathology or hepatitis and viral load was undetectable or greatly reduced in lungs following challenge with SARS-CoV. Altogether our results indicate that a recombinant trimeric S protein was able to elicit an efficacious protective immune response in vivo and warrant concern in the safety evaluation of a human vaccine against SARS-CoV.
Url:
DOI: 10.1016/j.vaccine.2006.08.011
PubMed: 17049691
Affiliations:
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Le document en format XML
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<term>Cricetinae</term>
<term>Dose-Response Relationship, Drug</term>
<term>Immunity, Mucosal</term>
<term>Immunoglobulin A (metabolism)</term>
<term>Immunoglobulin G (metabolism)</term>
<term>Membrane Glycoproteins (immunology)</term>
<term>Mice</term>
<term>Receptors, IgG (metabolism)</term>
<term>SARS Virus (immunology)</term>
<term>Severe Acute Respiratory Syndrome (prevention & control)</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Viral Envelope Proteins (immunology)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Anticorps antiviraux (immunologie)</term>
<term>Cricetinae</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires (immunologie)</term>
<term>Immunité muqueuse</term>
<term>Immunoglobuline A (métabolisme)</term>
<term>Immunoglobuline G (métabolisme)</term>
<term>Lymphocytes B (métabolisme)</term>
<term>Protéines de l'enveloppe virale (immunologie)</term>
<term>Relation dose-effet des médicaments</term>
<term>Récepteurs du fragment Fc des IgG (métabolisme)</term>
<term>Souris</term>
<term>Syndrome respiratoire aigu sévère ()</term>
<term>Virus du SRAS (immunologie)</term>
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<term>Membrane Glycoproteins</term>
<term>Viral Envelope Proteins</term>
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<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term>
<term>Glycoprotéines membranaires</term>
<term>Protéines de l'enveloppe virale</term>
<term>Virus du SRAS</term>
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<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term>
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<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>B-Lymphocytes</term>
<term>Immunoglobulin A</term>
<term>Immunoglobulin G</term>
<term>Receptors, IgG</term>
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<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Immunoglobuline A</term>
<term>Immunoglobuline G</term>
<term>Lymphocytes B</term>
<term>Récepteurs du fragment Fc des IgG</term>
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<term>Cricetinae</term>
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<term>Immunity, Mucosal</term>
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<term>Relation dose-effet des médicaments</term>
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<front><div type="abstract" xml:lang="en">Vaccine-induced antibodies can prevent or, in the case of feline infectious peritonitis virus, aggravate infections by coronaviruses. We investigated whether a recombinant native full-length S-protein trimer (triSpike) of severe acute respiratory syndrome coronavirus (SARS-CoV) was able to elicit a neutralizing and protective immune response in animals and analyzed the capacity of anti-S antibodies to mediate antibody-dependent enhancement (ADE) of virus entry in vitro and enhancement of replication in vivo. SARS-CoV-specific serum and mucosal immunoglobulins were readily detected in immunized animals. Serum IgG blocked binding of the S-protein to the ACE2 receptor and neutralized SARS-CoV infection in vitro. Entry into human B cell lines occurred in a FcgammaRII-dependent and ACE2-independent fashion indicating that ADE of virus entry is a novel cell entry mechanism of SARS-CoV. Vaccinated animals showed no signs of enhanced lung pathology or hepatitis and viral load was undetectable or greatly reduced in lungs following challenge with SARS-CoV. Altogether our results indicate that a recombinant trimeric S protein was able to elicit an efficacious protective immune response in vivo and warrant concern in the safety evaluation of a human vaccine against SARS-CoV.</div>
</front>
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<affiliations><list><country><li>République populaire de Chine</li>
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<tree><noCountry><name sortKey="Altmeyer, Ralf" sort="Altmeyer, Ralf" uniqKey="Altmeyer R" first="Ralf" last="Altmeyer">Ralf Altmeyer</name>
<name sortKey="Cheung, Yan Chung" sort="Cheung, Yan Chung" uniqKey="Cheung Y" first="Yan Chung" last="Cheung">Yan Chung Cheung</name>
<name sortKey="Chu, Shui Ling" sort="Chu, Shui Ling" uniqKey="Chu S" first="Shui Ling" last="Chu">Shui Ling Chu</name>
<name sortKey="Guarner, Jeannette" sort="Guarner, Jeannette" uniqKey="Guarner J" first="Jeannette" last="Guarner">Jeannette Guarner</name>
<name sortKey="Kien, Francois" sort="Kien, Francois" uniqKey="Kien F" first="François" last="Kien">François Kien</name>
<name sortKey="Lamirande, Elaine W" sort="Lamirande, Elaine W" uniqKey="Lamirande E" first="Elaine W" last="Lamirande">Elaine W. Lamirande</name>
<name sortKey="Nal, Beatrice" sort="Nal, Beatrice" uniqKey="Nal B" first="Béatrice" last="Nal">Béatrice Nal</name>
<name sortKey="Peiris, Malik" sort="Peiris, Malik" uniqKey="Peiris M" first="Malik" last="Peiris">Malik Peiris</name>
<name sortKey="Roberts, Anjeanette" sort="Roberts, Anjeanette" uniqKey="Roberts A" first="Anjeanette" last="Roberts">Anjeanette Roberts</name>
<name sortKey="Subbarao, Kanta" sort="Subbarao, Kanta" uniqKey="Subbarao K" first="Kanta" last="Subbarao">Kanta Subbarao</name>
<name sortKey="Tse, Jane" sort="Tse, Jane" uniqKey="Tse J" first="Jane" last="Tse">Jane Tse</name>
<name sortKey="Vogel, Leatrice" sort="Vogel, Leatrice" uniqKey="Vogel L" first="Leatrice" last="Vogel">Leatrice Vogel</name>
<name sortKey="Zaki, Sherif R" sort="Zaki, Sherif R" uniqKey="Zaki S" first="Sherif R" last="Zaki">Sherif R. Zaki</name>
</noCountry>
<country name="République populaire de Chine"><noRegion><name sortKey="Kam, Yiu Wing" sort="Kam, Yiu Wing" uniqKey="Kam Y" first="Yiu Wing" last="Kam">Yiu Wing Kam</name>
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